Collection includes materials related southern California tourism, the Mission Revival movement, peace movement (1st half 20th century, citrus industry, and materials related to specific collections,including fine art, statuary, bells, crosses, dolls, aviation, Native American basketry, and Asian arts.

The Homestead Museum collection includes a diverse array of items from Los Angeles and the Los Angeles Region covering the general period of 1830 to 1930 and includes ephemera, maps, photographs, printed works, and three-dimensional everyday artifacts. The Homestead Museum is a participant in the LAAS and ONE Archives Hidden Histories project.