Collections include pamphlets and ephemera documenting the growth, industries, transportation, and social organizations of Los Angeles and greater southern California; photograph collections; thousands of books related to southern California; and numerous archival collections related to indiviuals, the citrus industry, and businesses in the Inland Empire.

Collection of acrical materials from many of the early and present schools of nursing in Southern California with emphasis on those in and around Los Angeles dating from 1853 to currency.

The Homestead Museum collection includes a diverse array of items from Los Angeles and the Los Angeles Region covering the general period of 1830 to 1930 and includes ephemera, maps, photographs, printed works, and three-dimensional everyday artifacts. The Homestead Museum is a participant in the LAAS and ONE Archives Hidden Histories project.